Keywords
Phytochemical screening
sensitivity test
agar-well diffusion
How to Cite
Abstract
Plectranthus esculentus N.E.Br. is a dicotyledonous perennial shrub that grows up to 2m tall and belongs to Lamiaceae family. Presently, there is dearth of publications on its Phytochemical and antibacterial analysis. The study was conducted on phytochemical and antibacterial analysis of the plant tubers. The aim was to evaluate the phytochemical constituents and antibacterial activity. The phytochemicals were qualitatively accessed by adopting the standard procedures, while the antibacterial activity was tested via agar-well diffusion method. The result of the phytochemical analysis revealed the presence of Phenols, flavonoids, terpenoids, steroids, saponins, alkaloids and tannins all in higher reactions. 500, 250, 125 and 62.5 µg/ml were used as the antibiotic concentrations with Ofloxacin 5 µg/ml as the positive control, against; S. aureus, E. coli and K. pneumoniae. S. aureus showed the highest value of inhibition zone at 500 µg/ml, while the lowest value was recorded on K. pneumoniae. At 250 µg/ml, highest and lowest values were recorded on E. coli and K. pneumoniae respectively. E. coli showed the highest activity at 125 µg/ml, while K. pneumoniae showed the lowest activity. At 62.5 µg/ml, highest and lowest values were recorded on E. coli and K. pneumoniae. This study therefore concludes that the ethanol tuber extract harbors various Phytochemicals. The presence of phyto-constituents is useful for treating different ailments and it also has a potential of providing useful drugs for human use. It therefore recommends tuber extract of P. esculentus should be used as natural antibiotic in place of the chemically-combined.
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